Thanks vlaiv
What you are saying is very helpful and this will hopefully get me to the bottom of the problem.
1. All subs were taken at -15oC. The camera sometimes fluctuates to -16oC but 1/2 a degree should make much difference.
2. Lights - night time, luminance filter
Darks - on scope in shed at night time with end cap on
Bias - on scope in shed at night time with end cap on
Flats - taken using a computer monitor set to white page, four sheets of white paper over end dew shield, held by a cardboard holder, scope about 40cm from screen, at right angles. Also used same luminance filter.
Flat Darks - on scope in shed at night with end cap on
Camera not moved between lights and flats.