Hi Adam, as above Sgpro won't debayer so always shows image in mono.
Darks are needed to remove fixed pattern noise and hot pixels. You'll still get a nice result without but I would use them. I find that bias works better to calibrate flats.
I also used gain 100 and gain 0 and have just gone back to 0 as the full well is about double that of gain 100. You'll end up with better star colour at 0.
As for offset, just make sure you're not clipping blacks, check the histogram is well seperated from the left hand side and if it isn't adjust up until it is and remember that if you change it you'll need to redo all your calibration.
I've experimented with various sub lengths from 150 seconds to 300 and find at f3.3 I get the best results at between 150-180.