Following with interest.
Yesterday I had the opportunity (first night in weeks) to play with my astro-stuff (in the presence of a bright moon) and took 3 hours of the Pacman Nebula split in 12x300s, 15x240s and 20x180s, all at ISO800. I wanted to use APT histogram to set the optimal exposure time, but all three tests seemed overexposed (further to the right than expected). Next cloudy night (tomorrow unfortunately) I'll have a go with the data integrating all subs in a single image. Maybe not very practical in this target, but a new technique to learn.
Regarding calibration frames, since I only use Flats and Bias (both ISO-dependent only) I will use a single set for all the subs.
If you use Darks, I believe you will need a set for every different exposure time.
Looking forward to hear from the experts.