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Any ideas what is causing the 2 vertical areas in this image?

Ive stacked it with and with out calibration files and they are still there, but I cant see them on a single sub?

The calibration files are the same I have used on other images and it doesnt show on them.

Any ideas anyone?

By the way this is a massive stretch to enhance the problem:)

post-16950-133877673688_thumb.jpg

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Ive calibrated them using just darks, just flats, combinations of both, with and without bias, and still get the same result. I shot another 3 hours worth last night and its still there, but I did some new flats as well...it doesnt show in the flats!!! Perhaps its ment to be there?

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