Odd pixel defect when photographing the Trifid

[Bessel61cygni]

Hello Everyone, 
This site has given me some great answers in the past and always happy to see what folks are doing. 
I'm hoping someone might recognize this defect in the image below and maybe point me in the right direction. I've also supplied information that may offer clues. Does anyone recognize this odd pixel defect in the closer detail? Some day I think I might start a catalogue of defects and aberrations lol...

William Optics ZenithStar 61mm
QHY 290C Cooled Color 

29 Lights 
Exposure=4 min 
Debayer Preview=Force GBRG
Output Format=FITS files (*.fits)
Colour Space=RAW16
Temperature=-18
Offset=6
Gain=6

50 Darks
12 Flats

SharpCap 
PDH2
Photoshop
Imagesplus
Deep Sky StackerTrifid close up.tiff

Trifid panned out.tiff

[Clarkey]

I'm not sure I know what pixel defect you mean? I looks a bit hazy or slightly out of focus, but I can't see a specific defect.

[michael8554]

That looks like GBRG isn't the correct debayer .

Michael