Thank you for the welcome and input.
Yes, the tilt is not the issue, i will have to deal with that sepperately, probably with a tilt-correcting adaptor, it is also in the Canon exposure.
I use the FWHM assistant to get the value a small as possible and then i lock down the focuser. I then re-check the value to make sure it is has not changed, if it has i re-focus and lock again, repeat until i have the smallest value possible. This is working just fine for my Canon and has always been my focusing method since i started using backyard canon/nikon. This rules out focuser slip and focus shift from locking down the focuser. I believe the focus mode uses the livewiev from the camera and sends it to the computer, but i am not completely sure how it works technically.
After this i do some test exposures - this is where the Nikon 40s exposure is from with the donut stars. The telescope sat outside for 30-40 minutes before i started polar alignemet and focusing, so i would guess at least 50min. to an hour of cooling down before the image was captured. This is extremely strange and i have not seen this before - this is my second season doing AP, so i am not completely new anymorem but not excactly an expert either this is just a mystery to me.