Your target and some of reference stars are saturated in this 16 bit  image (several pixels with 65520 values).  I also told you earlier that you  had saturated stars in your 8 bit images (several pixels with 255 values).  The exposure you need will vary from night to night depending on atmospheric conditions and will also vary through the night as the star changes in height above the horizon.  I recommend checking some images before you start a run and adjusting your exposure  to limit the